An animal model consisting of tumor-bearing Msh2loxP/loxP;TgTg (Vil1-cre) mice treated with an anti-PD-1 and anti-LAG-3 combination showed reduced circulating Tregs and lower levels of Tex cells positive for CTLA-4, LAG-3, and PD-1 [112]. The gene discussed is PDCD1; the disease is neoplasm.