Both CD4+ and CD8+ T cells induced Ki67 in a flow cytometric analysis of pre- and post-therapy PBMC samples from 48 MM patients and mouse melanoma models treated with CTLA-4 and PD-1 blockade combinations and monotherapy [49], with the Ki67+ cells exhibiting a CD45RO+ memory phenotype. This evidence concerns the gene PDCD1 and Miyoshi myopathy.