Our data collectively suggest that: (1) age significantly influences the expression of genes related to AD onset and progression; (2) Gfap, Cst7, and Trem2 are the most promising biomarkers in both the PFC and the HPC from 5xFAD mice; (3) dysregulation of inflammatory miRNAs and genes is more pronounced at 9 months of age in the 5xFAD mice; (4) correlations between gene and miRNA expression levels suggest dynamic specificity dependent on genotype, age, and region. Here, TREM2 is linked to Alzheimer disease.