PTHLH and Cachexia: Given that NuF effectively inhibits the expression of PTHrP and ameliorates fat wasting (Figure 2B) and that PTHrP is a mediator of AT browning and tumor-induced cachexia, the protein and mRNA levels of lipolytic enzymes and thermogenic effectors involved in the browning process needed to be known in the first place (Figure 4A,B).