Lastly, considering the significant therapeutic effect of Sox9 ablation in advanced Akt-YAP1 cHCC-CCA, similar to our Sox9 CKO (acute deletion) data but contrasting with Sox9 LKO (developmental deletion) in a preventive manner, there is a need to revisit tumor studies conducted using developmental Cre strains-mediated gene KO systems, which may overlook adaptation. The gene discussed is YAP1; the disease is neoplasm.