Indeed, numerous studies have provided evidence supporting this theory by inducing HC-derived cHCC-CCA using the HC-specific co-expression of proto-oncogenes and biliary lineage commitment genes, such as myristoylated Akt (Akt) and constitutive-active YAP1 or NRAS [9,10,11,12]. This evidence concerns the gene AKT1 and cholangiocarcinoma.