The 3D-bioprinted culture of GBM cells resulted in significantly increased expressions of RARRES2 (chemerin), CXCL8, CXCL3, CCL20, CXCL2, CCL3, CCL4, and CXCL13 with a concomitant decrease in the expressions of CXCL14, CXCL12, and CX3CL1 compared to those in the spheroid culture. This evidence concerns the gene CCL20 and glioblastoma.