In a prospective multicenter study, the diagnostic performances of the secreted glycoprotein anosmin 1 (ANOS1), a component of the extracellular matrix; of the dihydropyrimidinase-like 3 (DPYSL3), a cell adhesion molecule involved in metastasis; and of MAGED2, related to the melanoma-associated antigen (MAGE) family involved in cancer development, were evaluated. Here, ANOS1 is linked to cancer.