Here, we employed two different subtypes of DLBCL cells, U2932 (ABC) and VAL (GCB), and intravenously injected them into three strains of immunodeficient mice, NOD-scid-IL2rgnull (NSG) mice expressing human IL6 (NSG-IL6), IL3/CSF2/KITLG (NSG-SGM3), or NSG mice expressing all four human cytokines (NSG-IL6/SGM3), to establish a pipeline for the rapid and reliable generation of in vivo DLBCL models. This evidence concerns the gene CSF2 and diffuse large B-cell lymphoma.