NFKB1 and melanoma: When we investigated the differentially represented cargo proteins in EVs secreted by Mel 270 and metastatic OMM 2.5 cells, we found that enrichment terms related to actin organization, cell–cell adhesion processes, cytoskeletal dynamics, and particularly, melanoma biology were among the most abundant components in EVs derived from Mel 270 cells, while the most overrepresented proteins in EVs from OMM 2.5 cells belonged to the MAPK, NF-kB, and Hedgehog signaling pathways, which may sustain the cell migration abilities already acquired by the metastatic cells.