This review delves into the multifaceted roles of KIF20A, ranging from its involvement in the transport of Golgi membranes and associated vesicles to its critical functions in mitosis, including the recruitment of Polo-like kinase 1 (PLK1) to the central spindle, the coordination with the chromosome passenger complex (CPC) during cytokinesis, and its role in activating the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) 3 pathway, which has significant implications for cancer progression. This evidence concerns the gene PLK1 and cancer.