The results further prove that a GABA-rich adzuki bean diet could inhibit glycogen decomposition, the gluconeogenesis rate-limiting enzyme G-6-Pase, and PEPCK activities in T2DM mice, suggesting that it promotes the glycolysis pathway in reverse and then regulates blood glucose concentration in T2DM mice and alleviates hyperglycemia symptoms in mice. This evidence concerns the gene PCK2 and type 2 diabetes mellitus.