In our study, we found that F1 in the maternal high-density treatments significantly increased the levels of IL-1β, IL-6, and TNF-α, reduced the level of IL-10 (Figure 7), accompanied by higher depressive and anxiety-like behaviors (Figure 6), lower immunity (Figure 8) and higher coccidial infection (Figure 9), which were in agreement with the above findings. Here, TNF is linked to Anxiety.