The majority of TAMs from both control and ACT tumors secreted inflammatory cytokines, as shown in the polyfunctional strength index plot in Fig. 6C. Expression of cytokines and chemokines by TAMs are shown as 2-D Tsne plots in Fig. 6D. Compared to the control tumor, TAMs from the ACT tumor had increased secretion of inflammatory factors including Tnfa, Mif, T-cell recruiting chemokine Ip-10, and Il-5, whereas decreased secretion of anti-inflammatory Il-10. The gene discussed is MIF; the disease is neoplasm.