In the old AD groups, we observed significantly lager IBA1% area in the MPO (Old-AD-Male vs Old-WT-Male: p = 0.0133; Old-AD-Female vs Old-WT-Female: p = 0.0319), LH, (Old-AD-Male vs Old-WT-Male: p = 0.0491; Old-AD-Female vs Old-WT-Female: p = 0.0480) and MM (Old-AD-Male vs Old-WT-Male: p = 0.0450; Old-AD-Female vs Old-WT-Female: p < 0.0001) (Fig. 2A, B). The gene discussed is PLOD1; the disease is Alzheimer disease.