With the alternative and more stringent SIRS criteria [13], resulting in a reduced septic group and increased nonseptic group, there was still a higher KC-like concentration in septic dogs with pyometra (238 pg/mL [133–698], p < 0.05) than in nonseptic dogs with pyometra (53.7 pg/mL [32.5–93.4], p < 0.0001) and healthy controls (24.6 pg/mL [12.3–37.4], pg/mL, p < 0.0001)(Fig. 1B). Here, CALCA is linked to systemic inflammatory response syndrome.