Cas9- and Cas12a-based biosensing can successfully detect EGFR T790M (93.9% sensitivity and 100% specificity) and L858R mutations in plasma and tissues from patients with lung cancer.155,190 Given the ability of CRISPR-based assays to detect SNPs and other mutations associated with specific cancers, cancer subtypes, and drug resistance phenotypes, there is significant potential for the development of CRISPR-based POCTs for common cancer-associated NA biomarkers. Here, EGFR is linked to cancer.