In the HFD group, the biomarker microbiota like Colidextribacter, Lachnospiraceae, Lachnospirales, and Dubosiella exhibited strong positive correlations with factors associated with obesity and fatty liver, such as body weight, triglycerides, PPARγ, and Srebp1. These correlations suggest that these microbiota may contribute to the development of obesity and its associated metabolic complications. The gene discussed is SREBF1; the disease is obesity due to melanocortin 4 receptor deficiency.