Within the CD8 positive T-cell populations, cluster C10 expressed exhaustion molecules, coexisting with T cell activation related molecules and tumor killing associated cytokine including IFN-γ, GZMB, TNFRSF9 (Figures 5C, E; Supplementary Table 8), we defined this cluster of cell as tumor-specific CTL, which is in agreement with previous cancer studies (23, 26, 27, 37). The gene discussed is TNFRSF9; the disease is cancer.