Next, we analyzed the frequencies of the top 20 differentially expressed genes (DEGs) in the HBV-ACLF versus NC/CHB/LC groups, identifying SEMA6B, PPARG, MERTK, ADAMTS2, and FAM20A as the top 5 significantly upregulated genes in the HBV-ACLF group (Figure 1D). This evidence concerns the gene ADAMTS2 and laryngotracheoesophageal cleft.