To investigate the effect of B7‐H3 on NPC cell behavior, we used RT‐qPCR and western blot analysis to screen for the most effective B7‐H3 shRNA (Figure 1A,B and Figure S1), and our data showed that sh‐B7‐H3‐RNA3 produced the greatest reduction in B7‐H3 mRNA and protein levels. This evidence concerns the gene CD276 and nasopharyngeal carcinoma.