Next, to further explore the mechanism of GCS1 in CRC, RNA sequencing was of HCT 116 cells in the GCS1 overexpression group and the control group (n = 3 replicates), and subsequent, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that GCS1 is involved mostly in protein processing in the ER, ubiquitin-mediated proteolysis, and the apoptotic pathway (Fig. 3A, Figure S4A). Here, MOGS is linked to colorectal carcinoma.