Then the adipose tissue macrophage was isolated and the cellular BCAA and BCKA were extracted for LC/MS/MS measurement, the results showed that in HFD-induced obesity mice, there were the accumulation of both BCAA (Leucine, Isoleucine and Valine) and BCKA (KIC, KMV and KIV) in adipose tissue macrophage (Fig. 1C, D). The gene discussed is ARID4B; the disease is Obesity.