ARID4B and obesity due to melanocortin 4 receptor deficiency: Then the adipose tissue macrophage was isolated and the cellular BCAA and BCKA were extracted for LC/MS/MS measurement, the results showed that in HFD-induced obesity mice, there were the accumulation of both BCAA (Leucine, Isoleucine and Valine) and BCKA (KIC, KMV and KIV) in adipose tissue macrophage (Fig. 1C, D).