In the serious stage of sepsis, age, sex percentage, pH, K+, Glu, Lac, HB, PLT, ALB, DBIL, IBIL, ALT, AST, TBA, SCR, BUN, UA, LDH, Ca2+, P2+, Mg2+, D-dimer, PCT, IL-2, IL-6, IL-10 and TNF-α were selected for multivariate logistic regression (Fig. 3b). The gene discussed is TNF; the disease is Sepsis.