Serum Lac, PLT, TBA, BUN, D-dimer, TNF-α levels of the early stage of sepsis, pH, Glu, ALB, TBA, CK-MB, PCT, and TNF-α levels of the serious stage of sepsis and complications with statistically significant differences were verified by the ROC curve (Fig. 4a–d). This evidence concerns the gene TNF and Sepsis.