This seems to coincide with our outcomes, showing that Sirt1 negatively correlates with the hepatic expression level of Il6 and Mcp1. Therefore, to some extent, it is likely that Sirt1 activation could be mediating the beneficial effects of exercise alone and especially in combination with n-3 PUFA supplementation on the prevention of MASLD/MASH associated to obesity and aging. This evidence concerns the gene SIRT1 and obesity due to melanocortin 4 receptor deficiency.