SIRT1 and metabolic dysfunction-associated steatotic liver disease: SIRT1 overexpression using adenovirus can reduce PGC-1α acetylation level and induced PPARα transcriptional signalling, as well as the expression of PPARα/PGC-1α targeting genes, thereby increasing FAO, and alleviating MASLD in mice with HFD-induced hepatic steatosis [66, 72].