CD8+ TILs/CD4+CD25+FOXP3+ Treg ratio was significantly correlated with T1 + 2 (28/57, 49.1%), lymph node metastasis (26/49, 53.1%), Stage I + II (29/61, 47.5%), and venous invasion (16/28, 57.1%) (Table 3). This evidence concerns the gene CD4 and metastatic malignant neoplasm in the lymph nodes.