In the present study, multiple TILs (CD4+ TILs, CD8+ TILs, CD25+ TILs, FOXP3+ TILs, CD4+CD25+FOXP3+ Tregs) and PD‐L1 expression were assessed in tissues specimens of patients with ESCC who underwent curative esophagectomy without any preoperative therapy to assess their clinicopathological characteristics and prognosis. The gene discussed is CD4; the disease is esophageal squamous cell carcinoma.