In the present study, we investigated CD4+ TILs, CD8+ TILs, CD25+ TILs, FOXP3+ TILs, CD4+CD25+FOXP3+ Tregs, and PD‐L1 expression status and their clinical significance in patients with ESCC. The gene discussed is FOXP3; the disease is esophageal squamous cell carcinoma.