In our study, green synthesized ZnO NPs caused ferroptosis in leukemia cells by disrupting redox homeostasis, decreasing the levels of TAC with elevated 10.13039/100007293TOS and intracellular iron levels, which was further supported by significantly elevating ACSL4, ALOX15, and decreasing SLC7A11 and GPx4 mRNA expressions. Here, ACSL4 is linked to leukemia.