Subsequently, we examined the expression of EAAT1 and EAAT2, and we found that the expression of both EAAT1 and EAAT2 was down-regulated in the brain of 3 × Tg-AD+OVX mice compared to 3 × Tg-AD mice (P = 0.0332 for Figure 8J; Figures 8A, C, D, H, I). This evidence concerns the gene SLC1A2 and Alzheimer disease.