Fasting blood samples from 67 participants (32 with PCOS, 35 controls) were collected before and after EXTE assays measured blood lipids, glucose, and insulin, including TC, TG, FFA, LDL-C, and HDL-C. Metabolite changes were detected via NTGC-MS, alongside clinical biochemical observations. The gene discussed is INS; the disease is polycystic ovary syndrome.