The tumor cytotoxicity of control molecules with single tumor binding domain was superior when the anti-TAA domain was closer to the anti-CD3ε domain (Fig. 2a,b); however, both trispecific molecules showed similar and potent tumor cell cytotoxicity, indicating that either of the two TAAs can be placed in close proximity to the anti-CD3ε domain for maximal cytotoxicity (Fig. 2c). The gene discussed is CD3E; the disease is neoplasm.