On day 2, tumor infiltrating CD8+ T cells had an activated phenotype showed by the elevated levels of CD69+ and CD25+ on T cells in ISB 2001-treated mice relative to both vehicle and CD3 × DU × DU (Fig. 7e,f), which was further supported by the elevated levels of effector cytokines (IFNγ and TNF) measured in the tumor supernatants (Fig. 7g). Here, TNF is linked to neoplasm.