To narrow down conserved differential transcripts resulting from the deletion of CBFβ, we performed RNA-seq analyses in both MC38 cells, and a second cancer cell line, E0771 (mouse breast cancer), and used Venn analysis to identify common differentially expressed genes (DEGs) between sgCbfb and sgNT cells across both cancer lines (Fig. 2A). The gene discussed is CBFB; the disease is cancer.