Given the role of ACSL4 in promoting lipid peroxidation, as a key fatty acid synthase in the ferroptosis pathway, particularly when antioxidant defenses are low [17], we created DU145 and C4-2B cell lines with ACSL4 knockdown (Fig. S4A, B), which led to a significant decrease in MDA and ROS levels in PCa cells (Fig. S4C, D). The gene discussed is FASN; the disease is posterior cortical atrophy.