Mrtfab- or Srf-null splenic T cells were harvested 3 days post-infection, when proliferation of OT-I WT and OT-I Mrtfab−/− cells was comparable, and cultured for 72 h either alone, with IL-2, or with IL-12, which allows maintenance of IL-2Rα expression51. Here, IL2 is linked to infection.