First, we assessed the efficacy of the transgenic pepck1 RNAi in flies, noted as pepck1RNAi, and found a significant decrease in pepck1 mRNA levels in the Ras/Src tumor-bearing strain when it was combined with the pepck1 RNAi line (Fig. 1A; Supplementary Fig. S2B). Here, PCK1 is linked to neoplasm.