These results suggest that pepck1 not only maintain trehalose homeostasis under NDS but also mediates HDS-induced elevation of trehalose levels in tumor cells through T6Pase. To further investigate whether pepck1 regulates glucose uptake, we measured uptake rates of Ras/Src tumor tissue exposed to the glucose analog 2NBDG for a controlled period of time. Here, PCK1 is linked to neoplasm.