In summary, the high expression of T‐cell‐related molecular markers CD3, CD4, CD8, CD45RO, B‐cell‐related molecular marker CD20, DC‐related molecular marker CD103, and B‐cell and DC‐related molecular marker CD11c is significantly correlated with TLS and gastrointestinal tumors. The gene discussed is CD4; the disease is digestive system neoplasm.