A total of 9 clustering modules were obtained in this study (Fig. 4), and the 9 large-scale clustering modules were #0 premature ovarian insufficiency (POI), #1fertility preservation, #2 follicular fluid, #3 human endometrial mesenchymal stem cell, #4 smad9 signaling pathway, #5 ovarian function, #6 female sexual dysfunction, #7 noncoding RNA and #8 cell fate regulator. The gene discussed is SMAD9; the disease is premature menopause.