Because PELP1 is an essential component of the Rix1 complex that facilitates the synthesis of new proteins, we investigated the impact of SMIP34 on new protein synthesis by treating HCC cells with different concentrations of SMIP34 and incubating them with puromycin for labeling of the C-terminus of translating polypeptide chains. The gene discussed is PELP1; the disease is hepatocellular carcinoma.