To further investigate the impact of PELP1 expression on the oncogenic capabilities of HCC cells, we augmented the expression of PELP1 in SNU475 and Hep3B cells which express relatively lower levels of PELP1 compared with other HCC cells by using a lentiviral vector (Supplementary Fig. S1A and S1B). Here, PELP1 is linked to hepatocellular carcinoma.