To investigate the effectof 5-H-Y on prostate cancer cell proliferation via AR signaling, we evaluated the mRNA expression levelsof the AR-responsive genes encoding PSA and transmembrane protease,serine 2 (TMPRSS2) after 5-H-Y treatment in LNCaP cells(Figure 3A,B). Here, TMPRSS2 is linked to Familial prostate cancer.