As DGUOK‐AS1/miR‐145‐5p/SIX1 axis regulates DNL, which plays an important role in modulating cancer cell proliferation and metastasis, we first examined if the DGUOK‐AS1/miR‐145‐5p/SIX1 axis regulates cancer cell proliferation and invasion in vitro. The gene discussed is DNASE2; the disease is cancer.