Importantly, SCD1 KD/ SCD1 KO almost abolished the ability of DGUOK‐AS1 overexpression, miR‐145‐5p inhibitor, or SIX1 overexpression to increase liver cancer cell proliferation and invasion, suggesting that SCD1 is critical for the regulation of cancer cell proliferation and invasion by DGUOK‐AS1/miR‐145‐5p/SIX1 axis. This evidence concerns the gene DGUOK and liver cancer.