Since the insulin/DGUOK‐AS1/miR‐145‐5p axis regulates SIX1 expression as well as liver cancer cell growth and metastasis, we investigated whether this axis affects histone acetylation at the SIX1 promoter and the recruitment of AIB1 and HBO1 to the DNL‐related gene promoters in cultured cells and liver tumor tissues. The gene discussed is DGUOK; the disease is liver cancer.