DNL has been shown to be mainly activated by the transcription factors SREBP‐1c, ChREBP, USFs, LXRs, and PPARγ.[3, 4, 5, 6, 7, 8] Using SIX1 KO hepatocarcinoma cell lines, SIX1 KO MEFs, SIX1 KO mice embryos, and tumor samples, we show that SIX1 regulates lipogenic gene expression, LDs, LCFA synthesis, and the production of TG and TC, facilitating tumor growth and metastasis. The gene discussed is MLXIPL; the disease is neoplasm.