The GO enrichment analysis, including BPs, CCs, and MFs, explained the function of the protein targets, and the KEGG pathway enrichment analysis suggested that 67 target proteins significantly enriched 150 signal pathways, including cancer-related pathways, such as the PI3K-AKT signaling pathway, Foxo signaling pathway, microRNAs in cancer, MAPK signaling pathway, HIF-1 signaling pathway, etc. In addition, the PPI network and cluster analysis indicated that CASP3, CTNNB1, JUN, IL6, MAPK3, HIF1A, STAT3, AKT1, TP53, CCND1, VEGFA, and EGFR may be vital to the effect of curcumin against NSCLC. This evidence concerns the gene HIF1A and cancer.