CD40LG and congenital syphilis: In contrast, serological, treponemal, and non-treponemal tests are used in clinical practice for the increasing prevalence of this pathology in pregnant women, with PCR being limited to research centers.21 This review included three articles with CSF analysis identifying Treponema by molecular tests.18,19,21 In two of these studies,18,19 presumptive cases of congenital syphilis with positive Western Blot IgM results were found.