Previous studies found that the hippocampus, cortex and hypothalamus regions of mice are associated with food intake or depression.[47, 48, 49, 50, 51, 52] To explore Atf4, and Znt3 participate in the vicious cycle of obesity and depression, we examined the mRNA expression levels of Wfs1, Atf4, and Znt3 in the hippocampus, cortex, and hypothalamus of WT and CKO mice (Figure S12A–C, Supporting Information). This evidence concerns the gene ATF4 and obesity due to melanocortin 4 receptor deficiency.