However, through in situ RNA-seq, we identified EMT as a prominent gene signature distinguishing Vim+ tumor cells from Vim− tumor cells and observed significantly increased expression of ZEB1, ZEB2, and CREB3L1 in Vim+ tumor cells relative to that in paired Vim− tumor cells within the sampled ROIs in the corresponding primary tumor section. Here, CREB3L1 is linked to neoplasm.