The earliest upregulation of ZEB1 during the initiation of the EMT process, the precise correlation between ZEB1 and VIM expression identified through scRNA-seq and immunofluorescence staining, and the reversal of EMT upon ZEB1 knockdown collectively confirm the pivotal role of ZEB1 in initiating and sustaining the EMT phenotype in breast cancer. Here, ZEB1 is linked to breast cancer.