TNF and hepatocellular carcinoma: To test this, we treated several HCC cell lines (PLC-8024, Hepa1-6, and H22) with TNF and IFNγ for 24 and 48 h and found that IFNγ, but not TNF, increased PRMT3 expression in a time-dependent manner (Fig. 2G, H and Supplementary Fig. 4E).