MYCN and neuroblastoma: When tested for protein expression, H2AFY was detected in a panel of human NB cell lines (Figure 2G) and we observed stronger H2AFY protein expression in MYCN-amplified NB cell lines (IMR32, SMS-KAN, SK-N-BE2, CHP134, and SK-N-DZ), compared with the nonamplified CHLA-15 and CHLA-20 lines (Figure 2G).