To determine the type of immune cells detecting endogenous DNA and causing autoimmunity in Trex1–/– mice, we generated three different conditional cGAS knockout mice on the Trex1–/– background: CD11ccre+ cGASFlox/Flox, LysMcre+ cGASFlox/Flox, and zDCcre+ cGASFlox/Flox mice with selective deletion of the cGAS gene in macrophages, dendritic cells (both pDC and cDC), and cDC, respectively. Here, CGAS is linked to Autoimmunity.