To understand the GLS1-NDUFB8 axis associated with glutamine flux in AA BLCA, we next treated BLCA cell lines with mitochondrial complex–specific inhibitors (IACS-010759 inhibits complex I; 3-nitropropionic acid inhibits complex II; oligomycin inhibits complex V; atovaquone inhibits complex III) (29–31) and measured GLS1 expression. Here, GLS is linked to bladder transitional cell carcinoma.