GLS and bladder transitional cell carcinoma: To investigate the race-specific mitochondria-mediated metabolic alterations, we analyzed the cytosolic and mitochondrial fractions of EA (UM-UC-3) and AA (UM-UC-1) BLCA cell lines and confirmed increased expression of GLS1 and NDUFB8 proteins in the mitochondrial fraction of AA BLCA compared with EA BLCA cell lines (Supplemental Figure 6D).