To investigate the race-specific mitochondria-mediated metabolic alterations, we analyzed the cytosolic and mitochondrial fractions of EA (UM-UC-3) and AA (UM-UC-1) BLCA cell lines and confirmed increased expression of GLS1 and NDUFB8 proteins in the mitochondrial fraction of AA BLCA compared with EA BLCA cell lines (Supplemental Figure 6D). Here, NDUFB8 is linked to bladder transitional cell carcinoma.