According to the subgroup analysis stratified by age, individuals younger than 20 years with higher ALT/AST were also more likely to develop NAFLD than those in other age groups: model 1 (OR = 22.528, 95% CI: 12.882, 39.399), model 2 (OR = 22.792, 95% CI: 12.680, 40.970) and model 3 (OR = 7.570, 95% CI: 3.515, 16.306). This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.