When ALT/AST ratio was converted from a continuous variable to a categorical variable (quartiles), participants in quartile 2 (ALT/AST 0.708-0.881), quartile 3 (ALT/AST 0.882-1.132) and quartile 4 (ALT/AST 1.133-3.314) were associated with 6.6%, 83%, 215.4% higher odds of being NAFLD, respectively, compared with quartile1 (ALT/AST 0.167-0.707). This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.