measured cytokine and matrix metalloproteinase (MMP) levels in breast fibroblasts overexpressing inhibitor of growth family member 1(ING1) and co-cultured these cells in three dimensions with Michigan Cancer Foundation - 7(MCF7) cells, finding that matrix ING1 expression could predict survival in ductal-type breast cancer patients, with high levels of ING1 in the matrix cells promoting breast cancer development by increasing MMP expression and downregulating tissue inhibitor of matrix metalloproteinases(TIMPs), potentially leading to reduced patient survival rates (58). The gene discussed is ING1; the disease is breast carcinoma.