To determine whether RTN4 plays a role in pyroptosis progression, we performed RTN4 silencing in osteosarcoma cell lines (U2OS, HOS, and 143B) and observed significant pyroptosis-associated “bubble” morphology by Annexin V-PI double staining (Figs. 2A and S3A). The gene discussed is ANXA5; the disease is osteosarcoma.