The normalized fraction of tumor area occupied by Ki67-positive cells was significantly lower in the TTX pre-treated group (68.02 ± 7.19) compared to the control group (100 ± 10.26), indicating reduced cell proliferation in response to TMZ treatment (n = 5, unpaired t-test, p = 0.034, Fig. 7G). This evidence concerns the gene MKI67 and neoplasm.