EIF2AK3 and central nervous system cancer: Immunostaining for pERK revealed a significant reduction in nuclear pERK intensity in the TTX pre-treated group (13.49 ± 8.4 mean nuclear fluorescent intensity per cell, n = 24) compared to the control group (8.347 ± 6.694 mean nuclear fluorescent intensity per cell, n = 24, p = 0.0153, Mann-Whitney test, Fig. 7D), suggesting that Nav channel blockade may reduce proliferative signaling pathways in glioma cells (Fig. 7D).