To determine if Merlin regulates Wnt signaling in meningioma cells, non-malignant M10G human meningioma cells, which encode NF2/Merlin28, were engineered to stably express CRISPR interference (CRISPRi) machinery (dCas9-KRAB)29 and transduced with non-targeted control sgRNAs (sgNTC) or sgRNAs suppressing endogenous NF2 (sgNF2) (Supplementary Fig. 4a). This evidence concerns the gene NF2 and meningioma.