APEX2 and cancer: To identify candidates mediating Merlin regulation of the Wnt pathway, M10G cells were transduced with doxycycline-inducible wildtype or cancer-associated missense Merlin constructs (L46R, A211D) that encoded C-terminal FLAG and APEX2 tags to enable subcellular localization experiments and proximity labeling proteomic mass spectrometry interrogation of candidate interactors31.