However, after meningioma cell stimulation with Wnt3a, (4) knockdown of endogenous NF2 resulted in a 50% loss of Tcf/Lef transcriptional activity (Fig. 2h, columns 4 vs 5), but (5) over-expression of exogenous NF2 either with endogenous Merlin (Fig. 2g, columns 4 vs 5) or without endogenous Merlin (Fig. 2h, columns 5 vs 6) increased Tcf/Lef transcriptional activity above endogenous levels. The gene discussed is NF2; the disease is meningioma.